An RNAi screen to identify proteins required for cohesion rejuvenation during meiotic prophase in Drosophila oocytes.

ABSTRACT: Accurate chromosome segregation during meiosis requires the maintenance of sister chromatid cohesion, initially established during premeiotic S phase. In human oocytes, DNA replication and cohesion establishment occur decades before chromosome segregation and deterioration of meiotic cohesion is one factor that leads to increased segregation errors as women age. Our previous work led us to propose that a cohesion rejuvenation program operates to establish new cohesive linkages during meiotic prophase in Drosophila oocytes and depends on the cohesin loader Nipped-B and the cohesion establishment factor Eco. In support of this model, we recently demonstrated that chromosome-associated cohesin turns over extensively during meiotic prophase and failure to load cohesin onto chromosomes after pr

SUBMITTER: Haseeb MA 

PROVIDER: S-EPMC11304968 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature