Mirror-assisted light-sheet microscopy: a simple upgrade to enable bi-directional sample excitation.

ABSTRACT:

Significance

Light-sheet microscopy is a powerful imaging technique that achieves optical sectioning via selective illumination of individual sample planes. However, when the sample contains opaque or scattering tissues, the incident light sheet may not be able to uniformly excite the entire sample. For example, in the context of larval zebrafish whole-brain imaging, occlusion by the eyes prevents access to a significant portion of the brain during common implementations using unidirectional illumination.

Aim

We introduce mirror-assisted light-sheet microscopy (mLSM), a simple inexpensive method that can be implemented on existing digitally scanned light-sheet setups by adding a single optical element-a mirrored micro-prism. The prism is placed near the sample to generate a

SUBMITTER: Zylbertal A 

PROVIDER: S-EPMC11304984 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature