High-throughput Oligopaint screen identifies druggable 3D genome regulators.

ABSTRACT: The human genome functions as a three-dimensional chromatin polymer, driven by a complex collection of chromosome interactions^1-3. Although the molecular rules governing these interactions are being quickly elucidated, relatively few proteins regulating this process have been identified. Here, to address this gap, we developed high-throughput DNA or RNA labelling with optimized Oligopaints (HiDRO)-an automated imaging pipeline that enables the quantitative measurement of chromatin interactions in single cells across thousands of samples. By screening the human druggable genome, we identified more than 300 factors that influence genome folding during interphase. Among these, 43 genes were validated as either increasing or decreasing interactions between topologically associating

SUBMITTER: Park DS 

PROVIDER: S-EPMC11305015 | biostudies-literature | 2023 Aug

REPOSITORIES: biostudies-literature