Nocardamine mitigates cellular dysfunction induced by oxidative stress in periodontal ligament stem cells.

ABSTRACT:

Background

The role of periodontal ligament stem cells (PDLSCs) in repairing periodontal destruction is crucial, but their functions can be impaired by excessive oxidative stress (OS). Nocardamine (NOCA), a cyclic siderophore, has been shown to possess anti-cancer and anti-bacterial properties. This study aimed to investigate the protective mechanisms of NOCA against OS-induced cellular dysfunction in PDLSCs.

Methods

The cytotoxicity of NOCA on PDLSCs was assessed using a CCK-8 assay. PDLSCs were then treated with hydrogen peroxide (H₂O₂) to induce OS. ROS levels, cell viability, and antioxidant factor expression were analyzed using relevant kits after treatment. Small molecule inhibitors U0126 and XAV-939 were employed to block ERK signaling and Wnt pa

SUBMITTER: He HP 

PROVIDER: S-EPMC11305061 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature