DNA stable isotope probing reveals the impact of trophic interactions on bioaugmentation of soils with different pollution histories.

ABSTRACT:

Background

Bioaugmentation is considered a sustainable and cost-effective methodology to recover contaminated environments, but its outcome is highly variable. Predation is a key top-down control mechanism affecting inoculum establishment, however, its effects on this process have received little attention. This study focused on the impact of trophic interactions on bioaugmentation success in two soils with different pollution exposure histories. We inoculated a ¹³C-labelled pollutant-degrading consortium in these soils and tracked the fate of the labelled biomass through stable isotope probing (SIP) of DNA. We identified active bacterial and eukaryotic inoculum-biomass consumers through amplicon sequencing of 16S rRNA and 18S rRNA genes coupled to a novel enrichment fac

SUBMITTER: Nieto EE 

PROVIDER: S-EPMC11305082 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature