Oncogenic transcription factors instruct promoter-enhancer hubs in individual triple negative breast cancer cells.

ABSTRACT: Sequencing-based mapping of ensemble pairwise interactions among regulatory elements support the existence of topological assemblies known as promoter-enhancer hubs or cliques in cancer. Yet, prevalence, regulators, and functions of promoter-enhancer hubs in individual cancer cells remain unclear. Here, we systematically integrated functional genomics, transcription factor screening, and optical mapping of promoter-enhancer interactions to identify key promoter-enhancer hubs, examine heterogeneity of their assembly, determine their regulators, and elucidate their role in gene expression control in individual triple negative breast cancer (TNBC) cells. Optical mapping of individual SOX9 and MYC alleles revealed the existence of frequent multiway interactions among promoters an

SUBMITTER: Zhao J 

PROVIDER: S-EPMC11305386 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature