Stem-loop and circle-loop TADs generated by directional pairing of boundary elements have distinct physical and regulatory properties.

ABSTRACT: The chromosomes in multicellular eukaryotes are organized into a series of topologically independent loops called TADs. In flies, TADs are formed by physical interactions between neighboring boundaries. Fly boundaries exhibit distinct partner preferences, and pairing interactions between boundaries are typically orientation-dependent. Pairing can be head-to-tail or head-to-head. The former generates a stem-loop TAD, while the latter gives a circle-loop TAD. The TAD that encompasses the Drosophila even skipped (eve) gene is formed by the head-to-tail pairing of the nhomie and homie boundaries. To explore the relationship between loop topology and the physical and regulatory landscape, we flanked the nhomie boundary region with two attP sites. The attP site

SUBMITTER: Ke W 

PROVIDER: S-EPMC11305674 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature