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Alternative dimethylsulfoniopropionate biosynthesis enzymes in diverse and abundant microorganisms.

ABSTRACT: Dimethylsulfoniopropionate (DMSP) is an abundant marine organosulfur compound with roles in stress protection, chemotaxis, nutrient and sulfur cycling and climate regulation. Here we report the discovery of a bifunctional DMSP biosynthesis enzyme, DsyGD, in the transamination pathway of the rhizobacterium Gynuella sunshinyii and some filamentous cyanobacteria not previously known to produce DMSP. DsyGD produces DMSP through its N-terminal DsyG methylthiohydroxybutyrate S-methyltransferase and C-terminal DsyD dimethylsulfoniohydroxybutyrate decarboxylase domains. Phylogenetically distinct DsyG-like proteins, termed DSYE, with methylthiohydroxybutyrate S-methyltransferase activity were found in diverse and environmentally abundant algae, comprising a mix of low, high and previously unknown DMSP producers. Algae containing DSYE, particularly bloom-forming Pelagophyceae species, were globally more abundant DMSP producers than those with previously described DMSP synthesis genes. This work greatly increases the number and diversity of predicted DMSP-producing organisms and highlights the importance of Pelagophyceae and other DSYE-containing algae in global DMSP production and sulfur cycling.

SUBMITTER: Wang J

PROVIDER: S-EPMC11306096 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

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Alternative dimethylsulfoniopropionate biosynthesis enzymes in diverse and abundant microorganisms.

Wang Jinyan J Curson Andrew R J ARJ Zhou Shun S Carrión Ornella O Liu Ji J Vieira Ana R AR Walsham Keanu S KS Monaco Serena S Li Chun-Yang CY Dong Qing-Yu QY Wang Yu Y Rivera Peter Paolo L PPL Wang Xiao-Di XD Zhang Min M Hanwell Libby L Wallace Matthew M Zhu Xiao-Yu XY Leão Pedro N PN Lea-Smith David J DJ Zhang Yu-Zhong YZ Zhang Xiao-Hua XH Todd Jonathan D JD

Nature microbiology 20240611 8

Dimethylsulfoniopropionate (DMSP) is an abundant marine organosulfur compound with roles in stress protection, chemotaxis, nutrient and sulfur cycling and climate regulation. Here we report the discovery of a bifunctional DMSP biosynthesis enzyme, DsyGD, in the transamination pathway of the rhizobacterium Gynuella sunshinyii and some filamentous cyanobacteria not previously known to produce DMSP. DsyGD produces DMSP through its N-terminal DsyG methylthiohydroxybutyrate S-methyltransferase and C- ...[more]

PMID: 38862603

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Alternative dimethylsulfoniopropionate biosynthesis enzymes in diverse and abundant microorganisms.

Publications

Alternative dimethylsulfoniopropionate biosynthesis enzymes in diverse and abundant microorganisms.

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