Overexpression of NLRP12 enhances macrophage immune response and alleviates herpes simplex keratitis.

ABSTRACT:

Introduction

Herpes simplex keratitis (HSK) is a blinding disease caused by corneal infection of Herpes simplex virus type 1 (HSV-1). Effective clearance of HSV-1 from the infected cornea is crucial for HSK management. Macrophages play an important part in the innate immune defense against viral infections. This study investigates the immunomodulatory role of NLRP12 in macrophage immune response during HSV-1 infection.

Methods

NLRP12 expression post-infection was assessed in various macrophage cell lines. Overexpression of NLRP12 was achieved by lentiviral transfection, and its effect on HSV-1 replication and immune responses were examined. Mechanistic insights into the role of NLRP12 were explored using immunofluorescence and Western Blot. For in vivo studies, ocular

SUBMITTER: Jiang J 

PROVIDER: S-EPMC11306119 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature