A self-inducible heterologous protein expression system in <i>Komagataella phaffii</i> (<i>Pichia pastoris</i>).

ABSTRACT: Komagataella phaffii (previously described as Pichia pastoris) is a yeast that produces high-level heterologous proteins with a wide range of applications in medicine and industry. The methanol-induced alcohol oxidase I promoter (P_AOX1) is frequently used for protein expression in this yeast. However, limitations on the use of methanol have been observed in large-scale production, including its flammability, toxicity, and need for special handling. Here, we propose to develop a system using recombinant cells constitutively expressing pectinmethyl esterase for expression of two reporter proteins, GFP and azurin, under the control of P_AOX1 using pectin in production medium. So, this system is coherent with yeast culture medium containing pectin and heterol

SUBMITTER: Unver Y 

PROVIDER: S-EPMC11306816 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature