GINS2 promotes the progression of human HNSCC by altering RRM2 expression.

ABSTRACT:

Introduction

GINS2 exerts a carcinogenic effect in multiple human malignancies, while it is still unclear that the potential roles and underlying mechanisms of GINS2 in HNSCC.

Methods

TCGA database was used to screen out genes with significant differences in expression in HNSCC. Immunohistochemistry and qRT-PCR were used to measure the expression of GINS2 in HNSCC tissues and cells. GINS2 was detected by qRT-PCR or western blot after knockdown or overexpression. Celigo cell counting, MTT, colony formation, and flow cytometric assay were used to check the ability of proliferation and apoptosis. Bioinformatics and microarray were used to screen out the downstream genes of GINS2.

Results

GINS2 in HNSCC tissues and cells was up-regulated, which was correlated with poor pr

SUBMITTER: Wang T 

PROVIDER: S-EPMC11307040 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature