Discovery and Validation of Methylated DNA Markers From Pancreatic Neuroendocrine Tumors.

ABSTRACT:

Background and aims

Methylated DNA markers (MDMs) accurately identify several different cancer types, but there are limited data for pancreatic neuroendocrine tumors (pNETs). We aimed to identify MDM candidates in tissue that differentiate pNETs from normal pancreas.

Methods

wUsing DNA from frozen normal pancreas (13) and pNET (51) tissues, we performed reduced representation bisulfite sequencing for MDM discovery. Validation in independent formalin fixed paraffin embedded tissues used pNET cases (67; solid = 50, cystic = 17), normal pancreas (24), and buffy coat (36) controls. Primary pNET MDM distributions were compared with lung (36), small bowel (36) NETs, and metastatic pNET (25) tissues. The discrimination accuracy was summarized as the area under the receiver operator

SUBMITTER: Majumder S 

PROVIDER: S-EPMC11307501 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature