Deletion of miR-150 Prevents Spontaneous T Cell Proliferation and the Development of Colitis.

ABSTRACT:

Background and aims

To examine the roles of microRNAs in the development of colitis, we conducted the RNA-sequencing studies using RNA derived from normal and colitogenic CD4+ T cells. Colitogenic CD4⁺ T cells demonstrated the increased expression of miR-150. We focused on the involvement of miR-150 in the colitis.

Methods

We crossed miR-150 knockout mice and T-cell-specific Rap1KO mice, which is colitis model mice and spontaneously develop the colitis with tubular adenomas in microbiota-dependent manner.

Results

MiR-150 silencing completely inhibited the expansion of pathogenic Th17 cells and the development of colitis.

Conclusion

MiR-150 is a potential therapeutic target of inflammatory bowel diseases.

SUBMITTER: Ishihara S 

PROVIDER: S-EPMC11308117 | biostudies-literature | 2023

REPOSITORIES: biostudies-literature