Characterization of GQA as a novel β-lactamase inhibitor of CTX-M-15 and KPC-2 enzymes.

ABSTRACT: β-lactam resistance is a significant global public health issue. Outbreaks of bacteria resistant to extended-spectrum β-lactams and carbapenems are serious health concerns that not only complicate medical care but also impact patient outcomes. The primary objective of this work was to express and purify two soluble recombinant representative serine β‑lactamases using Escherichia coli strain as an expression host and pET101/D as a cloning vector. Furthermore, a second objective was to evaluate the potential, innovative, and safe use of galloylquinic acid (GQA) from Copaifera lucens as a potential β-lactamase inhibitor.In the present study, bla_CTX-M-15 and bla_KPC-2 represented genes encoding for serine β-lactamases that were cloned from parent isolates of E. coli and K.

SUBMITTER: Al-Madboly LA 

PROVIDER: S-EPMC11308155 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature