The polyunsaturated fatty acid docosahexaenoic affects mitochondrial function in prostate cancer cells.

ABSTRACT:

Background

Prostate cancer (PCa) shows a rewired metabolism featuring increased fatty acid uptake and synthesis via de novo lipogenesis, both sharply related to mitochondrial physiology. The docosahexaenoic acid (DHA) is an omega-3 polyunsaturated fatty acid (PUFA) that exerts its antitumoral properties via different mechanisms, but its specific action on mitochondria in PCa is not clear. Therefore, we investigated whether the DHA modulates mitochondrial function in PCa cell lines.

Methods

Here, we evaluated mitochondrial function of non-malignant PNT1A and the castration-resistant (CRPC) prostate 22Rv1 and PC3 cell lines in response to DHA incubation. For this purpose, we used Seahorse extracellular flux assay to assess mitochondria function, [¹⁴C]-glucose to eva

SUBMITTER: Tamarindo GH 

PROVIDER: S-EPMC11308158 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature