Impact of reference design on estimating SARS-CoV-2 lineage abundances from wastewater sequencing data.

ABSTRACT:

Background

Sequencing of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA from wastewater samples has emerged as a valuable tool for detecting the presence and relative abundances of SARS-CoV-2 variants in a community. By analyzing the viral genetic material present in wastewater, researchers and public health authorities can gain early insights into the spread of virus lineages and emerging mutations. Constructing reference datasets from known SARS-CoV-2 lineages and their mutation profiles has become state-of-the-art for assigning viral lineages and their relative abundances from wastewater sequencing data. However, selecting reference sequences or mutations directly affects the predictive power.

Results

Here, we show the impact of a mutation- and sequence-

SUBMITTER: Aßmann E 

PROVIDER: S-EPMC11308188 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature