Acidic preconditioning induced intracellular acid adaptation to protect renal injury via dynamic phosphorylation of focal adhesion kinase-dependent activation of sodium hydrogen exchanger 1.

ABSTRACT:

Background

Disruptions in intracellular pH (pH_i) homeostasis, causing deviations from the physiological range, can damage renal epithelial cells. However, the existence of an adaptive mechanism to restore pH_i to normalcy remains unclear. Early research identified H⁺ as a critical mediator of ischemic preconditioning (IPC), leading to the concept of acidic preconditioning (AP). This concept proposes that short-term, repetitive acidic stimulation can enhance a cell's capacity to withstand subsequent adverse stress. While AP has demonstrated protective effects in various ischemia-reperfusion (I/R) injury models, its application in kidney injury remains largely unexplored.

Methods

An AP model was established in human kidney (HK2) cells by treatin

SUBMITTER: Chen A 

PROVIDER: S-EPMC11308338 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature