Microfluidic measurement of intracellular mRNA with a molecular beacon probe towards point-of-care radiation triage.

ABSTRACT: In large-scale radiation exposure events, the ability to triage potential victims by the received radiation dosage is crucial. This can be evaluated by radiation-induced biological changes. Radiation-responsive mRNA is a class of biomarkers that has been explored for dose-dependency with methods such as RT-qPCR. However, these methods are challenging to implement for point-of-care devices. We have designed and used molecular beacons as probes for the measurement of radiation-induced changes of intracellular mRNA in a microfluidic device towards determining radiation dosage. Our experiments, in which fixed TK6 cells labeled with a molecular beacon specific to BAX mRNA exhibited dose-dependent fluorescence in a manner consistent with RT-qPCR analysis, demonstrate that such intracellul

SUBMITTER: Meng X 

PROVIDER: S-EPMC11308381 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature