Paneth Cell Secretion in vivo Requires Expression of Tmem16a and Tmem16f.

ABSTRACT:

Background and aims

Paneth cells play a central role in intestinal innate immune response. These cells are localized at the base of small intestinal crypts of Lieberkuhn. The calcium-activated chloride channel TMEM16A and the phospholipid scramblase TMEM16F control intracellular Ca²⁺ signaling and exocytosis. We analyzed the role of TMEM16A and TMEM16F for Paneth cells secretion.

Methods

Mice with intestinal epithelial knockout of Tmem16a (Tmem16a^-/-) and Tmem16f (Tmem16f^-/-) were generated. Tissue structures and Paneth cells were analyzed, and Paneth cell exocytosis was examined in small intestinal organoids in vitro. Intracellular Ca²⁺ signals were measured and were compared between wild-type and Tmem16 knockout mice. Bact

SUBMITTER: Schreiber R 

PROVIDER: S-EPMC11308424 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature