Characterization of a novel MgtE homolog and its structural dynamics in membrane mimetics.

ABSTRACT: Magnesium (Mg²⁺) is the most abundant divalent cation in the cell and is critical for numerous cellular processes. Despite its importance, the mechanisms of intracellular Mg²⁺ transport and its regulation are poorly understood. MgtE is the main Mg²⁺ transport system in almost half of bacterial species and is an ortholog of mammalian SLC41A1 transporters, which are implicated in neurodegenerative diseases and cancer. To date, only MgtE from Thermus thermophilus (MgtE_TT) has been extensively characterized, mostly in detergent micelles, and gating-related structural dynamics in biologically relevant membranes are scarce. The MgtE homolog from Bacillus firmus (MgtE_BF) is unique since it lacks the entire Mg²⁺-sensing N-domain but

SUBMITTER: Brahma R 

PROVIDER: S-EPMC11309985 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature