Macrophage OTUD1-CARD9 axis drives isoproterenol-induced inflammatory heart remodelling.

ABSTRACT:

Background

Chronic inflammation contributes to the progression of isoproterenol (ISO)-induced heart failure (HF). Caspase-associated recruitment domain (CARD) families are crucial proteins for initiation of inflammation in innate immunity. Nonetheless, the relevance of CARDs in ISO-driven cardiac remodelling is little explored.

Methods

This study utilized Card9^-/- mice and reconstituted C57BL/6 mice with either Card9^-/- or Otud1^-/- marrow-derived cells. Mechanistic studies were conducted in primary macrophages, cardiomyocytes, fibroblasts and HEK-293T cells.

Results

Here, we demonstrated that CARD9 was substantially upregulated in murine hearts infused with ISO. Either whole-body CARD9 knockout or myeloid-specific CARD9 deletion inhibi

SUBMITTER: Qian J 

PROVIDER: S-EPMC11310286 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature