Testosterone Inhibits Lipid Accumulation in Porcine Preadipocytes by Regulating ELOVL3.

ABSTRACT: Castration is commonly used to reduce stink during boar production. In porcine adipose tissue, castration reduces androgen levels resulting in metabolic disorders and excessive fat deposition. However, the underlying detailed mechanism remains unclear. In this study, we constructed porcine preadipocyte models with and without androgen by adding testosterone exogenously. The fluorescence intensity of lipid droplet (LD) staining and the fatty acid synthetase (FASN) mRNA levels were lower in the testosterone-treated cells than in the untreated control cells. In contrast, the mRNA levels of adipose triglycerides lipase (ATGL) and androgen receptor (AR) were higher than in the testosterone-treated cells than in the control cells. Subsequently, transcriptomic sequencing of p

SUBMITTER: Xie F 

PROVIDER: S-EPMC11310965 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature