Applying Spatial Metabolomics To Investigate Age- and Drug-Induced Neurochemical Changes.

ABSTRACT: In an era when population aging is increasing the burden of neurodegenerative conditions, deciphering the mechanisms underlying brain senescence is more important than ever. Here, we present a spatial metabolomics analysis of age-induced neurochemical alterations in the mouse brain using negative ionization mode mass spectrometry imaging. The age-dependent effects of the acetylcholinesterase inhibitor tacrine were simultaneously examined. For ultrahigh mass resolution analysis, we utilized a Fourier-transform ion cyclotron resonance spectrometer. To complement this, a trapped ion mobility spectrometry time-of-flight analyzer provided high speed and lateral resolution. The chosen approach facilitated the detection and identification of a wide range of metabolites, from amino acids to sphing

SUBMITTER: Vallianatou T 

PROVIDER: S-EPMC11311129 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature