Regulatory B Cells Expressing Granzyme B from Tolerant Renal Transplant Patients: Highly Differentiated B Cells with a Unique Pathway with a Specific Regulatory Profile and Strong Interactions with Immune System Cells.

ABSTRACT: The aim of our study was to determine whether granzyme B-expressing regulatory B cells (GZMB⁺ B cells) are enriched in the blood of transplant patients with renal graft tolerance. To achieve this goal, we analysed two single-cell RNA sequencing (scRNAseq) datasets: (1) peripheral blood mononuclear cells (PBMCs), including GZMB⁺ B cells from renal transplant patients, i.e., patients with stable graft function on conventional immunosuppressive treatment (STA, n = 3), drug-free tolerant patients (TOL, n = 3), and patients with antibody-mediated rejection (ABMR, n = 3), and (2) ex-vivo-induced GZMB⁺ B cells from these groups. In the patient PBMCs, we first showed that natural GZMB⁺ B cells were enriched in genes specific to Natur

SUBMITTER: Sailliet N 

PROVIDER: S-EPMC11311295 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature