Dynamics of Replication-Associated Protein Levels through the Cell Cycle.

ABSTRACT: The measurement of dynamic changes in protein level and localization throughout the cell cycle is of major relevance to studies of cellular processes tightly coordinated with the cycle, such as replication, transcription, DNA repair, and checkpoint control. Currently available methods include biochemical assays of cells in bulk following synchronization, which determine protein levels with poor temporal and no spatial resolution. Taking advantage of genetic engineering and live-cell microscopy, we performed time-lapse imaging of cells expressing fluorescently tagged proteins under the control of their endogenous regulatory elements in order to follow their levels throughout the cell cycle. We effectively discern between cell cycle phases and S subphases based on fluorescence intensity and

SUBMITTER: Atemin A 

PROVIDER: S-EPMC11311332 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature