Transcriptional and epigenetic characterization of a new in vitro platform to model the formation of human pharyngeal endoderm.

ABSTRACT:

Background

The Pharyngeal Endoderm (PE) is an extremely relevant developmental tissue, serving as the progenitor for the esophagus, parathyroids, thyroids, lungs, and thymus. While several studies have highlighted the importance of PE cells, a detailed transcriptional and epigenetic characterization of this important developmental stage is still missing, especially in humans, due to technical and ethical constraints pertaining to its early formation.

Results

Here we fill this knowledge gap by developing an in vitro protocol for the derivation of PE-like cells from human Embryonic Stem Cells (hESCs) and by providing an integrated multi-omics characterization. Our PE-like cells robustly express PE markers and are transcriptionally homogenous and similar to in vivo mouse PE cel

SUBMITTER: Cipriano A 

PROVIDER: S-EPMC11312149 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature