A Review of Probe-Based Enrichment Methods to Inform Plant Virus Diagnostics.

ABSTRACT: Modern diagnostic techniques based on DNA sequence similarity are currently the gold standard for the detection of existing and emerging pathogens. Whilst individual assays are inexpensive to use, assay development is costly and carries risks of not being sensitive or specific enough to capture an increasingly diverse range of targets. Sequencing can provide the entire nucleic acid content of a sample and may be used to identify all pathogens present in the sample when the depth of coverage is sufficient. Targeted enrichment techniques have been used to increase sequence coverage and improve the sensitivity of detection within virus samples, specifically, to capture sequences for a range of different viruses or increase the number of reads from low-titre virus infections. Vertebrate viruse

SUBMITTER: Farrall T 

PROVIDER: S-EPMC11312432 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature