Magnetic Stirring Device for Limiting the Sedimentation of Cells inside Microfluidic Devices.

ABSTRACT: In experiments considering cell handling in microchannels, cell sedimentation in the storage container is a key problem because it affects the reproducibility of the experiments. Here, a simple and low-cost cell mixing device (CMD) is presented; the device is designed to prevent the sedimentation of cells in a syringe during their injection into a microfluidic channel. The CMD is based on a slider crank device made of 3D-printed parts that, combined with a permanent magnet, actuate a stir bar placed into the syringe containing the cells. By using A549 cell lines, the device is characterized in terms of cell viability (higher than 95%) in different mixing conditions, by varying the oscillation frequency and the overall mixing time. Then, a dedicated microfluidic experiment is designed to ev

SUBMITTER: Cremaschini S 

PROVIDER: S-EPMC11314744 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature