Identification and experimental verification of immune-related hub genes in intervertebral disc degeneration.

ABSTRACT:

Background

Inflammation and immune factors are the core of intervertebral disc degeneration (IDD), but the immune environment and epigenetic regulation process of IDD remain unclear. This study aims to identify immune-related diagnostic candidate genes for IDD, and search for potential pathogenesis and therapeutic targets for IDD.

Methods

Gene expression datasets were obtained from the Gene Expression Omnibus (GEO). Differential expression immune genes (Imm-DEGs) were identified through weighted gene correlation network analysis (WGCNA) and linear models for microarray data analysis (Limma). LASSO algorithm was used to identify feature genes related to IDD, which were compared with core node genes in PPI network to obtain hub genes. Based on the coefficients of hub genes, a

SUBMITTER: Huang Z 

PROVIDER: S-EPMC11315086 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature