A noninvasive photoactivatable split-Cre recombinase system for genome engineering in zebrafish.

ABSTRACT: The cyclic recombinase (Cre)/loxP recombination system is a powerful technique for in vivo cell labeling and tracking. However, achieving high spatiotemporal precision in cell tracking using this system is challenging due to the requirement for reliable tissue-specific promoters. In contrast, light-inducible systems offer superior regional confinement, tunability, and non-invasiveness compared to conventional lineage-tracing methods. Here, we took advantage of the unique strengths of the zebrafish to develop an easy-to-use highly efficient, genetically encoded, magnets-based, light-inducible transgenic Cre/loxP system. We demonstrate that our system does not exhibit phototoxicity or leakiness in the dark, and it enables efficient and robust Cre/loxP recombinatio

SUBMITTER: Elsaid R 

PROVIDER: S-EPMC11315165 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature