RNAseq analysis of oocyte maturation from the germinal vesicle stage to metaphase II in pig and human.

ABSTRACT: During maturation oocytes at the germinal vesicle (GV) stage progress to metaphase II (MII). However, during in vitro maturation a proportion often fail to progress. To understand these processes, we employed RNA sequencing to examine the transcriptome profile of these three groups of oocytes from the pig. We compared our findings with similar public oocyte data from humans. The transcriptomes in oocytes that failed to progress was similar to those that did. We found in both species, the most upregulated genes in MII oocytes were associated with chromosome segregation and cell cycle processes, while the most down regulated genes were relevant to ribosomal and mitochondrial pathways. Moreover, those genes involved in chromosome segregation during GV to MII transition were conserved in pig a

SUBMITTER: Tang F 

PROVIDER: S-EPMC11315340 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature