Intervertebral Disc-on-a-Chip^MF: A New Model for Mouse Disc Culture via Integrating Mechanical Loading and Dynamic Media Flow.

ABSTRACT: This study aims to develop an ex vivo organ-on-a-chip model, intervertebral Disc-on-a-Chip^MF, to investigate integrated effects of mechanical loading and nutrition on disc health. The system consists of a detachable multilayer microfluidic chip, a Computer-Arduino-based control system, and a mechanical loading unit, which were optimized for accurate axial force measurement and the maintenance of a 21-day ex vivo disc culture. To ensure accuracy of axial force, we optimized the axial mechanical loading regimen, used the Computer-Arduino-based system and low-profile force sensors (LPFS) to control the mechanical loading unit, and modeled the force distribution by using computational simulation. A 21-day ex vivo disc culture was demonstrated using the Disc-on-a

SUBMITTER: Xie W 

PROVIDER: S-EPMC11315454 | biostudies-literature | 2023 Nov

REPOSITORIES: biostudies-literature