Differential transcriptional invasion signatures from patient derived organoid models define a functional prognostic tool for head and neck cancer.

ABSTRACT: Clinical outcome for patients suffering from HPV-negative head and neck squamous cell carcinoma (HNSCC) remains poor. This is mostly due to highly invasive tumors that cause loco-regional relapses after initial therapeutic intervention and metastatic outgrowth. The molecular pathways governing the detrimental invasive growth modes in HNSCC remain however understudied. Here, we have established HNSCC patient derived organoid (PDO) models that recapitulate 3-dimensional invasion in vitro. Single cell mRNA sequencing was applied to study the differences between non-invasive and invasive conditions, and in a collective versus single cell invading PDO model. Differential expression analysis under invasive conditions in Collagen gels reveals an overall upregulation of a YAP-centered transcriptio

SUBMITTER: Haughton PD 

PROVIDER: S-EPMC11315671 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature