Comparative characterisation of extracellular vesicles from canine and human plasma: a necessary step in biomarker discovery.

ABSTRACT: Extracellular Vesicles (EV) have become an interesting focus as novel biomarkers of disease and are increasingly reported upon in humans and other species. The Minimal Information for Studies of Extracellular Vesicles 2018 (MISEV2018) guidelines were published to improve rigor and standardisation within the EV field and provide a framework for the reliable isolation and characterisation of EV populations. However, this rigor and standardisation has been challenging in the area of comparative medicine. Herein we present the successful isolation of EVs from human and canine plasma using Size Exclusion Chromatography and characterise these EVs according to best international practice. This study provides evidence for the reliable comparison of human and canine EVs isolated by this approach, a

SUBMITTER: Bollard SM 

PROVIDER: S-EPMC11315736 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature