Establishing a method for the cryopreservation of viable peripheral blood mononuclear cells in the International Space Station.

ABSTRACT: The analysis of cells frozen within the International Space Station (ISS) will provide crucial insights into the impact of the space environment on cellular functions and properties. The objective of this study was to develop a method for cryopreserving blood cells under the specific constraints of the ISS. In a ground experiment, mouse blood was directly mixed with a cryoprotectant and gradually frozen at -80 °C. Thawing the frozen blood sample resulted in the successful recovery of viable mononuclear cells when using a mixed solution of dimethylsulfoxide and hydroxyethyl starch as a cryoprotectant. In addition, we developed new freezing cases to minimize storage space utilization within the ISS freezer. Finally, we confirmed the recovery of major mononuclear immune cell subsets from the

SUBMITTER: Ishii H 

PROVIDER: S-EPMC11315897 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature