Autophagy-mediated ID1 turnover dictates chemo-resistant fate in ovarian cancer stem cells.

ABSTRACT:

Background

The mechanisms enabling dynamic shifts between drug-resistant and drug-sensitive states in cancer cells are still underexplored. This study investigated the role of targeted autophagic protein degradation in regulating ovarian cancer stem cell (CSC) fate decisions and chemo-resistance.

Methods

Autophagy levels were compared between CSC-enriched side population (SP) and non-SP cells (NSP) in multiple ovarian cancer cell lines using immunoblotting, immunofluorescence, and transmission electron microscopy. The impact of autophagy modulation on CSC markers and differentiation was assessed by flow cytometry, immunoblotting and qRT-PCR. In silico modeling and co-immunoprecipitation identified ID1 interacting proteins. Pharmacological and genetic approaches along with An

SUBMITTER: Phadte P 

PROVIDER: S-EPMC11316295 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature