MiR-1915-3p regulates megakaryocytic and erythroid differentiation by targeting SOCS4.

ABSTRACT:

Background

Proper control of the lineage bias of megakaryocytic and erythroid progenitor cells (MEPs) is of significant importance, the disorder of which will lead to abnormalities in the number and function of platelets and erythrocytes. Unfortunately, the signaling pathways regulating MEP differentiation largely remain to be elucidated. This study aimed to analyze the role and the underlying molecular mechanism of miR-1915-3p in megakaryocytic and erythroid differentiation.

Methods

We utilized miRNA mimics and miRNA sponge to alter the expression of miR-1915-3p in megakaryocytic and/or erythroid potential cells; siRNA and overexpression plasmid to change the expression of SOCS4, a potential target of miR-1915-3p. The expression of relevant surface markers was detected by f

SUBMITTER: Yuan X 

PROVIDER: S-EPMC11316338 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature