Drug library screening against metronidazole-sensitive and metronidazole-resistant Trichomonas vaginalis isolates.

ABSTRACT:

Objectives

Metronidazole and tinidazole are effective treatments for most patients with trichomoniasis but not for individuals who are infected with very resistant strains of Trichomonas vaginalis or persons with hypersensitivity to the 5-nitroimidazole drugs. Thus, there is a need for additional oral therapies to treat trichomoniasis.

Methods

We screened the US Drug Collection Library against metronidazole-susceptible and resistant strains of T vaginalis. Activity was measured by incubating parasites and drugs for 48 h in the presence of tritiated thymidine. Growth inhibition was determined by the reduction of incorporated radioactivity by compounds at 20 μM in comparison to media control. Drugs that showed good initial activity were further tested to calculate IC50 values.

SUBMITTER: Goodhew EB 

PROVIDER: S-EPMC11316563 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature