<i>Promethearchaeum syntrophicum</i> gen. nov., sp. nov., an anaerobic, obligately syntrophic archaeon, the first isolate of the lineage 'Asgard' archaea, and proposal of the new archaeal phylum <i>Promethearchaeota</i> phyl. nov. and kingdom <i>Promethearchaeati</i> regn. nov.

ABSTRACT: An anaerobic, mesophilic, syntrophic, archaeon strain MK-D1^T, was isolated as a pure co-culture with Methanogenium sp. strain MK-MG from deep-sea methane seep sediment. This organism is, to our knowledge, the first cultured representative of 'Asgard' archaea, an archaeal group closely related to eukaryotes. Here, we describe the detailed physiology and phylogeny of MK-D1^T and propose Promethearchaeum syntrophicum gen. nov., sp. nov. to accommodate this strain. Cells were non-motile, small cocci, approximately 300-750 nm in diameter and produced membrane vesicles, chains of blebs and membrane-based protrusions. MK-D1^T grew at 4-30 °C with optimum growth at 20 °C. The strain grew chemoorganotrophically with amino acids, peptides and yeast extrac

SUBMITTER: Imachi H 

PROVIDER: S-EPMC11316595 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature