Simple protocol for combined extraction of exocrine secretions and RNA in small arthropods.

ABSTRACT: The integration of data from multiple sources and analytical techniques to obtain novel insights and answer challenging questions is a hallmark of modern science. In arthropods, exocrine secretions may act as pheromones, defensive substances, antibiotics, as well as surface protectants, and as such they play a crucial role in ecology and evolution. Exocrine chemical compounds are frequently characterized by gas chromatography-mass spectrometry. Technological advances of recent years now allow us to routinely characterize the total gene complement transcribed in a particular biological tissue, often in the context of experimental treatment, via RNAseq. We here introduce a novel methodological approach to successfully characterize exocrine secretions and full transcriptomes of one and

SUBMITTER: Frohlich D 

PROVIDER: S-EPMC11316613 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature