Exploring the effects of three-finger toxins from Naja ashei venom on neuronal and immunological cancer cell membranes.

ABSTRACT: Three-finger proteins are the most abundant toxins in the venom of Naja ashei, a snake species from the Elapidae family. This research aimed to describe the effects of varying charges of these proteins, isolated from Naja ashei venom using SEC and IEX chromatography. The study examined how differently charged three-finger toxin fractions interact with and affect neuroblastoma (SK-N-SH) and promyeloblast (HL-60) cells, as well as model Langmuir membranes and liposomes designed to mimic cellular lipid composition. Findings revealed that protein surface charges significantly impact cell survival (MTT assay), membrane damage (lactate dehydrogenase release, malondialdehyde formation), and the structural and electrochemical properties of model membranes (Langmuir membranes and zeta potential for

SUBMITTER: Dyba B 

PROVIDER: S-EPMC11316797 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature