From promoter motif to cardiac function: a single DPE motif affects transcription regulation and organ function in vivo.

ABSTRACT: Transcription initiates at the core promoter, which contains distinct core promoter elements. Here, we highlight the complexity of transcriptional regulation by outlining the effect of core promoter-dependent regulation on embryonic development and the proper function of an organism. We demonstrate in vivo the importance of the downstream core promoter element (DPE) in complex heart formation in Drosophila. Pioneering a novel approach using both CRISPR and nascent transcriptomics, we show the effects of mutating a single core promoter element within the natural context. Specifically, we targeted the downstream core promoter element (DPE) of the endogenous tin gene, encoding the Tinman transcription factor, a homologue of human NKX2-5 associated with congenital heart diseases. The 7 bp subs

SUBMITTER: Sloutskin A 

PROVIDER: S-EPMC11317100 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature