The relationship between nanoscale genome organization and gene expression in mouse embryonic stem cells during pluripotency transition.

ABSTRACT: During early development, gene expression is tightly regulated. However, how genome organization controls gene expression during the transition from naïve embryonic stem cells to epiblast stem cells is still poorly understood. Using single-molecule microscopy approaches to reach nanoscale resolution, we show that genome remodeling affects gene transcription during pluripotency transition. Specifically, after exit from the naïve pluripotency state, chromatin becomes less compacted, and the OCT4 transcription factor has lower mobility and is more bound to its cognate sites. In epiblast cells, the active transcription hallmark, H3K9ac, decreases within the Oct4 locus, correlating with reduced accessibility of OCT4 and, in turn, with reduced expression of Oct4 nascent RNAs. Despite the high va

SUBMITTER: Garate X 

PROVIDER: S-EPMC11317139 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature