Stable isotope-resolved metabolomics analyses of metabolic phenotypes reveal variable glutamine metabolism in different patient-derived models of non-small cell lung cancer from a single patient.

ABSTRACT:

Introduction

Stable isotope tracers have been increasingly used in preclinical cancer model systems, including cell culture and mouse xenografts, to probe the altered metabolism of a variety of cancers, such as accelerated glycolysis and glutaminolysis and generation of oncometabolites. Comparatively little has been reported on the fidelity of the different preclinical model systems in recapitulating the aberrant metabolism of tumors.

Objectives

We have been developing several different experimental model systems for systems biochemistry analyses of non-small cell lung cancer (NSCLC¹) using patient-derived tissues to evaluate appropriate models for metabolic and phenotypic analyses.

Methods

To address the issue of fidelity, we have carried out a detailed St

SUBMITTER: Kinslow CJ 

PROVIDER: S-EPMC11317205 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature