Cellular dynamics in pig-to-human kidney xenotransplantation.

ABSTRACT:

Background

Xenotransplantation of genetically engineered porcine organs has the potential to address the challenge of organ donor shortage. Two cases of porcine-to-human kidney xenotransplantation were performed, yet the physiological effects on the xenografts and the recipients' immune responses remain largely uncharacterized.

Methods

We performed single-cell RNA sequencing (scRNA-seq) and longitudinal RNA-seq analyses of the porcine kidneys to dissect xenotransplantation-associated cellular dynamics and xenograft-recipient interactions. We additionally performed longitudinal scRNA-seq of the peripheral blood mononuclear cells (PBMCs) to detect recipient immune responses across time.

Findings

Although no hyperacute rejection signals were detected, scRNA-seq analyses

SUBMITTER: Pan W 

PROVIDER: S-EPMC11317223 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature