Multi-omics analysis of overexpressed tumor-associated proteins: gene expression, immunopeptide presentation, and antibody response in oropharyngeal squamous cell carcinoma, with a focus on cancer-testis antigens.

ABSTRACT:

Introduction

The human leukocyte antigen complex (HLA) is essential for inducing specific immune responses to cancer by presenting tumor-associated peptides (TAP) to T cells. Overexpressed tumor associated antigens, mainly cancer-testis antigens (CTA), are outlined as essential targets for immunotherapy in oropharyngeal squamous cell carcinoma (OPSCC). This study assessed the degree to which presentation, gene expression, and antibody response (AR) of TAP, mainly CTA, are correlated in OPSCC patients to evaluate their potential as immunotherapy targets.

Materials and methods

Snap-frozen tumor (N_Ligand/RNA=40), healthy mucosa (N_RNA=6), and healthy tonsils (N_Ligand=5) samples were obtained. RNA-Seq was performed using Illumina HiSeq 2500/NovaSe

SUBMITTER: Abou Kors T 

PROVIDER: S-EPMC11317303 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature