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Identification and characterization of a small-molecule metallophore involved in lanthanide metabolism.

ABSTRACT: Many bacteria secrete metallophores, low-molecular-weight organic compounds that bind ions with high selectivity and affinity, in order to access essential metals from the environment. Previous work has elucidated the structures and biosynthetic machinery of metallophores specific for iron, zinc, nickel, molybdenum, and copper. No physiologically relevant lanthanide-binding metallophore has been discovered despite the knowledge that lanthanide metals (Ln) have been revealed to be essential cofactors for certain alcohol dehydrogenases across a diverse range of phyla. Here, we report the biosynthetic machinery, the structure, and the physiological relevance of a lanthanophore, methylolanthanin. The structure of methylolanthanin exhibits a unique 4-hydroxybenzoate moiety which has not previously been described in other metallophores. We find that production of methylolanthanin is required for normal levels of Ln accumulation in the methylotrophic bacterium Methylobacterium extorquens AM1, while overexpression of the molecule greatly increases bioaccumulation and adsorption. Our results provide a clearer understanding of how Ln-utilizing bacteria sense, scavenge, and store Ln; essential processes in the environment where Ln are poorly bioavailable. More broadly, the identification of this lanthanophore opens doors for study of how biosynthetic gene clusters are repurposed for additional functions and the complex relationship between metal homeostasis and fitness.

SUBMITTER: Zytnick AM

PROVIDER: S-EPMC11317620 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature

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Identification and characterization of a small-molecule metallophore involved in lanthanide metabolism.

Zytnick Alexa M AM Gutenthaler-Tietze Sophie M SM Aron Allegra T AT Reitz Zachary L ZL Phi Manh Tri MT Good Nathan M NM Petras Daniel D Daumann Lena J LJ Martinez-Gomez Norma Cecilia NC

Proceedings of the National Academy of Sciences of the United States of America 20240730 32

Many bacteria secrete metallophores, low-molecular-weight organic compounds that bind ions with high selectivity and affinity, in order to access essential metals from the environment. Previous work has elucidated the structures and biosynthetic machinery of metallophores specific for iron, zinc, nickel, molybdenum, and copper. No physiologically relevant lanthanide-binding metallophore has been discovered despite the knowledge that lanthanide metals (Ln) have been revealed to be essential cofac ...[more]

PMID: 39078674

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Dataset Information

Identification and characterization of a small-molecule metallophore involved in lanthanide metabolism.

Publications

Identification and characterization of a small-molecule metallophore involved in lanthanide metabolism.

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