Fluorinated trehalose analogues for cell surface engineering and imaging of Mycobacterium tuberculosis.

ABSTRACT: The sensitive, rapid and accurate diagnosis of Mycobacterium tuberculosis (Mtb) infection is a central challenge in controlling the global tuberculosis (TB) pandemic. Yet the detection of mycobacteria is often made difficult by the low sensitivity of current diagnostic tools, with over 3.6 million TB cases missed each year. To overcome these limitations there is an urgent need for next-generation TB diagnostic technologies. Here we report the use of a discrete panel of native ¹⁹F-trehalose (F-Tre) analogues to label and directly visualise Mtb by exploiting the uptake of fluorine-modified trehalose analogues via the mycobacterial trehalose LpqY-SugABC ATP-binding cassette (ABC) importer. We discovered the extent of modified F-Tre uptake correlates with

SUBMITTER: Guy CS 

PROVIDER: S-EPMC11317875 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature