Paired organoids from primary gastric cancer and lymphatic metastasis are useful for personalized medicine.

ABSTRACT:

Background

Organoids are approved by the US FDA as an alternative to animal experiments to guide drug development and for sensitivity screening. Stable organoids models of gastric cancer are desirable for personalized medicine and drug screening.

Methods

Tumor tissues from a primary cancer of the stomach and metastatic cancer of the lymph node were collected for 3D culture. By long-term culture for over 50 generations in vitro, we obtained stably growing organoid lines. We analyzed short tandem repeats (STRs) and karyotypes of cancer cells, and tumorigenesis of the organoids in nude mice, as well as multi-omics profiles of the organoids. A CCK8 method was used to determine the drugs sensitivity to fluorouracil (5-Fu), platinum and paclitaxel.

Results

Paired organoid l

SUBMITTER: Yang R 

PROVIDER: S-EPMC11318189 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature