Single and few cell analysis for correlative light microscopy, metabolomics, and targeted proteomics.

ABSTRACT: The interactions of proteins, membranes, nucleic acid, and metabolites shape a cell's phenotype. These interactions are stochastic, and each cell develops differently, making it difficult to synchronize cell populations. Consequently, studying biological processes at the single- or few-cell level is often necessary to avoid signal dilution below the detection limit or averaging over many cells. We have developed a method to study metabolites and proteins from a small number of or even a single adherent eukaryotic cell. Initially, cells are lysed by short electroporation and aspirated with a microcapillary under a fluorescent microscope. The lysate is placed on a carrier slide for further analysis using liquid-chromatography mass spectrometry (LC-MS) and/or reverse-phase protein (RPPA) appr

SUBMITTER: Rima L 

PROVIDER: S-EPMC11318241 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature